Part:BBa_K4397005
ssDNA1
Tau Aptamer: ssDNA1
Target: Tau 381 and Tau 410 monomers
Binding Affinity: 190 nM (tau 381) and 350 nm (tau 410) (capillary electrophoresis)
Usage and Biology
Data shows that with growing concentration of tau in the equilibrium mixture, the peak of tau–ssDNA1 complex increases, while that of free ssDNA1 decreases [1]. Secondly, when investigated using NECEEM, the complexes of tau with ssDNA2 were present in the amount much lower than those of tau with ssDNA1 and similarly, the complexes of tau with ssDNA3 were undetectable with the used concentration of tau [1]. The Kd value of the tau–ssDNA1 complex is lower than those of the tau–ssDNA2 and the tau–ssDNA3 complexes by more than two orders of magnitude [1]. The difference in the affinity for the three ssDNA molecules indicates that tau binds ssDNA in a sequence-specific fashion [1].
[1] Krylova, S. M., Musheev, M., Nutiu, R., Li, Y., Lee, G., & Krylov, S. N. (2005). Tau protein binds single-stranded DNA sequence specifically–the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures. FEBS letters, 579(6), 1371-1375.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |